Actin associated with purified lymphocyte plasma membrane
نویسندگان
چکیده
منابع مشابه
Phosphorylated lymphocyte plasma-membrane proteins.
Lymphocytes were labelled by incubation with [32P]Pi and their plasma membranes isolated. Analysis by one-dimensional and two-dimensional gel electrophoresis revealed a small number of strongly phosphorylated polypeptides. Two of these were especially prominent; they had molecular weights of about 52000 and 90000, were acidic and were apparently not glycosylated. Similar patterns were obtained ...
متن کاملPolyamines and neomycin inhibit the purified plasma-membrane Ca2+ pump by interacting with associated polyphosphoinositides.
We investigated the effect of spermine, spermidine, putrescine and neomycin on the activity of the plasma-membrane Ca2+ pump and on its stimulation by negatively charged phospholipids and calmodulin. Millimolar concentrations of spermine and to a lesser extent of spermidine decreased the ATPase activity in the presence of phosphatidylinositol 4,5-bisphosphate (PIP2), without affecting the stimu...
متن کاملLymphocyte Products and the Macrophage Plasma Membrane
Infection of the mouse peritoneal cavity by bacillus Calmette-Gu6rin (BCG) 1 markedly alters the surface properties of macrophages (mob), compard with cells obtained from uninfected control animals or after injection of thioglycollate broth. BCG-activated peritoneal mob (BCG-PM) express enhanced Ia antigens (Ag), but reduced receptors for mannose-terminated glycoconjugates (MFR), Fc receptors, ...
متن کاملPurified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion
CD2 is a T lymphocyte glycoprotein that functions in adhesion of T lymphocytes and also as a putative receptor for activation signals. Functional data suggest that LFA-3, a widely distributed cell surface glycoprotein, may be the biological ligand of CD2. We have purified LFA-3 from human erythrocytes and characterized the purified protein functionally. LFA-3 bound specifically to CD2+ cells, a...
متن کاملPreparation of inside-out vesicles of pig lymphocyte plasma membrane.
Between 30 and 50% of pig lymphocyte plasma membrane vesicles were not bound by concanavalin A (Con A)-Sepharose. Various results suggest that the Con A-unretarded fraction represents "inside-out" membrane vesicles. First, an alternative cell surface ligand, anti-lymphocytic serum, gave a similar fractionation to Con A. Second, lack of binding by Con A was not due to lack of carbohydrate or to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1976
ISSN: 0014-5793
DOI: 10.1016/0014-5793(76)80507-8